Inside a Shell—Organometallic Catalysis Inside Encapsulin Nanoreactors

Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins self-assembling protein-based nanocompartments from the prokaryotic repertoire that present highly attractive platform intracellular compartmentalization by design. Using single-molecule Förster resonance energy transfer and 3D-MINFLUX analysis, we analyze fluorescently labeled encapsulins on basis. Furthermore, equipping these capsules with synthetic ruthenium catalyst via covalent attachment to non-native host protein, able perform in vitro catalysis go show engineered can be used as hosts transition metal living confined space.